Description of service

Analytical services "Structural Integrity". Set of basic assays to address a vaccine candidate antigens size, purity, quantity assembly status (multimers and/or aggregates), and integrity using analytical PAGE, SEC, FPLC/HPLC methods.

Timeline 

2 months

Contact 

Holger Spiegel

holger.spiegel@ime.fraunhofer.de

0049241608512461

Services do NOT include

Costs for shipment of samples will be the responsibility of the User.

Possible Output

Report summarizing the results obtained in the different assays regarding protein a) purity, b) size, c) integrity, d) assembly status, and quantity

Sample Requirements - input of users

Purified protein samples either in solution or lyophilized/freeze-dried. High concentrations are helpful (1mg/ml). Minimum amount for complete comprehensive analysis 500µ-1mg.Sequences, biophysical properties, storage buffer compositions and further available information on stability.

Lead Scientist

Holger Spiegel

Single compound analysis (structural, integrity, purity, binding)

Free 

Publications

  • Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. (DOI: 10.1111/pbi.12255)

  • Production, Quality Control, Stability and Pharmacotoxicity of a Malaria Vaccine Comprising Three Highly Similar PfAMA1 Protein Molecules to Overcome Antigenic Variation (DOI:10.1371/journal.pone.0164053)