Description of service

Analytical services "Structural Integrity". Set of basic assays to address a vaccine candidate antigens size, purity, quantity assembly status (multimers and/or aggregates), and integrity using analytical PAGE, SEC, FPLC/HPLC methods.


2 months


Holger Spiegel


Services do NOT include

Costs for shipment of samples will be the responsibility of the User.

Possible Output

Report summarizing the results obtained in the different assays regarding protein a) purity, b) size, c) integrity, d) assembly status, and quantity

Sample Requirements - input of users

Purified protein samples either in solution or lyophilized/freeze-dried. High concentrations are helpful (1mg/ml). Minimum amount for complete comprehensive analysis 500µ-1mg.Sequences, biophysical properties, storage buffer compositions and further available information on stability.

Lead Scientist

Holger Spiegel

Single compound analysis (structural, integrity, purity, binding)



  • Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. (DOI: 10.1111/pbi.12255)

  • Production, Quality Control, Stability and Pharmacotoxicity of a Malaria Vaccine Comprising Three Highly Similar PfAMA1 Protein Molecules to Overcome Antigenic Variation (DOI:10.1371/journal.pone.0164053)

This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N° 730964.

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