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Embryonic Stem Cells

Flow Cytometry​

Application Deadline: 

Call dates coming soon

Dates:

-

Location:

Fontenay-aux-roses, France​

Contact:

Dr. Anne-Sophie Gallouet
anne-sophie.gallouet@cea.fr

Provider:

IDMIT Infrastructure (CEA)

IDMIT Infrastructure (CEA)

Administrative support:

For questions about the application process, registration and scholarships: transvacinfo@euvaccine.eu

Registration fees

-

Minimal requirements 

Basic flow cytometry knowledge (3 to 4 colour experiments).

Who should apply

Anyone with the minimal requirements and wishing to improve flow knowledge.

Learning outcomes

To master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.

Course content

DAY-1 (9:00 am to 5:30 pm)

1. Introduction to the course
2. Optimization of protocols for intracellular staining of immune cells (ICS): from the choice of the markers to controls
3. Presentation of the ICS protocol and ICS hands on session Cell stimulation Fixation and Permeabilization
4. Sample Acquisition Course Introduction to DIVA Introduction to FlowJo Quality controls Staining and Acquisition



DAY-2 (8:30 am to 12:30)
5. Data analysis hands on session Sample analysis using FlowJo Post sample analysis using SPICE
6. Storage, analysis and visualization of Flow Cytometry data

Overview

The course will comprise lectures of experts in the field presenting methods for assessing the immune response in human and animal models. In particular, it will be focused on combined staining of surface markers and intracellular markers (cytokines and degranulation markers), allowing the characterization of activation, differentiation and functionality of lymphoid and myeloid cells. It will include approaches to master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results

Scholarships

We are pleased to offer free registration and travel funding to selected researchers from low- and middle-income countries (LMICs). These registration and travel grants for LMIC applicants are generously sponsored by the Coalition for Epidemic Preparedness Innovations (CEPI).

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Please note that not all courses are open for application submission, check open calls to know which for which courses you can apply for at the moment.

Be the first to know about the upcoming application calls, follow European Vaccine Initiative on X and LinkedIn or subscribe to EVI Newsletter.

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