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Multiplex cytokine/chemokine analysis
Free
Services do NOT include
Costs for shipment of samples.
Possible Output
Raw data in electronic format (i.e .xlsx o .xls); graphs of results if requested.
Sample Requirements - input of users
Serum, plasma and tissue culture supernatants (10 ul for sera and plasma samples, 50 ul for culture supernatant).
Lead Scientist
Donata Medaglini
Description of service
UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay. Both technologies allow the simultaneous detection of multiple factors in serum, plasma and tissue culture supernatants. UNISI will receive murine or human samples from users. Multiple biomarkers, such as cytokines, chemokines and growth factors will be detected using one of the available multiplex system to test up to 46 murine and 53 human analysts. Users will decide how many proteins will be tested and the analysis will be carried out by our technicians. Data analysis can be also performed.
Timeline
3 days, from sample delivery to assay
2 days, for data analysis, if requested
15-30 days, if particular panels are requested
Publications
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Ciabattini A, et al. Modulation of Primary Immune Response by Different Vaccine Adjuvants. Front Immunol. 2016 Oct 17;7:427. eCollection 2016.
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Fiorino F et al. Prime-boost strategies in mucosal immunization affect local IgA production and the type of th response. Front Immunol. 2013 May 29;4:128. doi: 10.3389/fimmu.2013.00128. eCollection 2013.
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