Construction of commensal bacterial strains displaying
Description of service
UNISI will provide access to the design and construction of commensal bacterial strains displaying selected antigens on their surface. S. gordonii is a human commensal bacterium which has been developed to deliver mucosal vaccines. A genetic system for stable chromosomal integration allows the expression of recombinant proteins on the bacterial surface. This system also allows the co-expression of two heterologous molecules on the surface of the same bacterial vector, i.e. vaccine antigens and adjuvant molecules or two different vaccine antigens.
3 months from receiving the sequence.
The recombinant strain, together with analysis of protein expression (i.e. western blot, or dot plot, or FACS analysis).
Sample Requirements - input of users
The sequence of the protein to express on the recombinant strain.
Pozzi G et al. Delivery and expression of a heterologous antigen on the surface of streptococci. Infect Immun. 1992 May;60(5):1902-7
Oggioni MR et al. Engineering the gram-positive cell surface for construction of bacterial vaccine vectors. Methods. 1999 Sep;19(1):163-73. Review
Oggioni MR, Pozzi G.A host-vector system for heterologous gene expression in Streptococcus gordonii.Gene. 1996 Feb 22;169(1):85-90