UNISI has established a NGS platform based on Ion Proton instrument (Thermo Fisher) for targeted RNA sequencing. Laminar flow cabinets are present in a BLS2 area for RNA extraction from blood. For RNA quality and quantity assessment we have a Qubit fluorimeter and an Agilent Bioanalyzer. Two advanced technologies for detecting and measuring multiple soluble biomarkers in soluble samples are also available in our facility, the Luminex xMap and flow cytometric CBA assay. Both systems can simultaneously detect many targets such as cytokines, chemokines and inflammatory biomarkers in a single sample that can be serum, plasma and tissue culture supernatants. Multiplexing analysis is especially useful when only a small amount of sample is available, maximizing the number of proteins that can be analysed. These technologies are particularly powerful when looking for changes in concentrations of multiple targets under specific conditions, such as following a vaccine administration, infection, or a drug treatment. The assays reduce sample requirements and time to results in comparison with traditional ELISA and Western blot techniques. The laboratory has also all of the technologies required to produce recombinant bacteria expressing vaccine antigens to be used as non-pathogenic vaccine vectors. The laboratory has all of the technologies required to perform molecular work including gradient polymerase chain reaction (PCR) blocks, RT-PCR and FACS. UNISI hosts a unit of Systems Biology and Bioinformatics, equipped with the necessary infrastructure for data storage and high-performance computational analyses. The main areas of activity of the unit are the analysis of NGS experiments, and the mathematical modelling of biological systems at the cellular and molecular level.