TNA or Training provided
VIM carries out virology and immunology research using large animals such as pigs (influenza) and ruminants (RSV). We propose cytometer analysis using up to 20 different parameters, Fluorescent microscopy, in vitro restimulation cultures, qRT-PCR analysis, ELISA and ELISPOT. We produce live and inactivated viruses in our BSL2 and BSL3 facilities, and perform immunogenicity and efficacy studies in pigs (influenza) and sheep (RSV) with a focus on skin vaccination and extended monitoring of humoral and cellular immune responses in the blood and lung. PFIE. All kind of projects using mice or farm animals can be performed, with most of animal and zoonotic pathogens up to BSL3. PFIE can supply SPF mice, SPF poultry, germfree chicks (chicken and quails), health controlled pigs and small ruminants as well as cattle and ponies. Various equipment is available in BSL3 containment like continuous telemetric monitoring of temperature in most species, fibro-, broncho- or laparoscopy for large and mid sized animals, ultrasonography, in vivo or ex vivo imaging in fluorescence and/or bio-luminescence: IVIS Spectrum® (Perkin Elmer) for small animals or organs, Cell Vizio® Dual Band confocal laser endomicroscopy (Mauna Kea Tech.), Fluobeam® (Fluoptics), surgery rooms, automated haematology and biochemistry analysers.