Course Goals 

To be introduced to multimodal in vivo imaging from the basic principles including basics in biophysics, methodologies for in vivo imaging, image analysis and biological interpretation of the results. Different imaging modalities will be addressed in these courses : PET-CT, In vivo two-photon microscopy and Confocal endomicroscopy, Histology. Course will comprise lectures from experts in the field, practical work, demonstrations and open discussions in collaboration with the Laboratory of Imaging the Infection and Immunity (L3i) core facility.

Timeline 

Application Deadline:  

15 November 2019

Course Dates:

2-4 March 2020 (3 days)

Q3 2021 

Contact 

Catherine Chapon catherine.chapon@cea.fr

Learning Outcomes 

To master multimodal imaging (PET-CT, In vivo two-photon microscopy and Confocal endomicroscopy, Histology) from the basic theory including basics in biophysics, methodologies for in vivo imaging, to image analysis and interpretation of the results.

Who should apply

Anyone wishing to be introduced to in vivo imaging (confocal, two-photon, PET-CT imaging) in NHP.

Minimal requirements

From beginners to Basic knowledge in imaging.

Accommodation

Accommodation is not covered, to be arranged by participants. Travel costs are also not included.

M8  In vivo Imaging

Course content

Day 1: PET-CT

  • Positron Emission Tomography– X Ray Computed Tomography (PET-CT)

  • Introduction to PET-CT technologies: basics in physics, choice of radiotracers and applications

  • Protocols for PET-CT imaging in NHP

  • Observation of experiments (with phantoms)

  • Image analysis (practical work)

Day 2: In vivo Two-Photon and confocal microscopy

  • Introduction to two-photon and confocal

  • microscopy technologies: basics in physics, choice of fluorophores and applications

  • Protocols for in vivo two-photon and confocal imaging in NHP

  • Observation of experiments (with phantoms)

  • Image analysis (practical work)

Day 3: Ex vivo analyses by histology

  • Ex vivo analyses Introduction to histology (immunohistofluorescence (IHF) and immunohistochimie (IHC))

  • Image analyses (practical work)