M7 Flow Cytometry
Course content
DAY-1 (9:00 am to 5:30 pm)
1. Introduction to the course
2. Optimization of protocols for intracellular staining of immune cells (ICS): from the choice of the markers to controls
3. Presentation of the ICS protocol and ICS hands on session Cell stimulation Fixation and Permeabilization
4. Sample Acquisition Course Introduction to DIVA Introduction to FlowJo Quality controls Staining and Acquisition
DAY-2 (8:30 am to 12:30)
5. Data analysis hands on session Sample analysis using FlowJo Post sample analysis using SPICE
6. Storage, analysis and visualization of Flow Cytometry data
Learning Outcomes
To master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.
Minimal requirements
Accommodation
Course Goals
The course will comprise lectures of experts in the field presenting methods for assessing the immune response in human and animal models. In particular, it will be focused on combined staining of surface markers and intracellular markers (cytokines and degranulation markers), allowing the characterization of activation, differentiation and functionality of lymphoid and myeloid cells. It will include approaches to master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.
Timeline
Application Deadline:
1 May 2019 / 1 May 2021
Course Dates:
18-19 September 2019/Q3 2021 (1,5 days)
Organiser
FlowCyTech laboratory, IDMIT Institute of Biology François JACOB, CEA, Fontenay-aux-roses, France
Participant Nr.: 12
Contact
Dr. Anne-Sophie Gallouet