Course content

DAY-1 (9:00 am to 5:30 pm)

1. Introduction to the course

2. Optimization of protocols for intracellular staining of immune cells (ICS): from the choice of the markers to controls

3. Presentation of the ICS protocol and ICS hands on session Cell stimulation Fixation and Permeabilization

4. Sample Acquisition Course Introduction to DIVA Introduction to FlowJo Quality controls Staining and Acquisition

DAY-2 (8:30 am to 12:30)

5. Data analysis hands on session Sample analysis using FlowJo Post sample analysis using SPICE

6. Storage, analysis and visualization of Flow Cytometry data

Learning Outcomes 

To master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.

Who should apply

Anyone with the minimal requirements and wishing to improve flow knowledge

Minimal requirements

Basic flow cytometry knowledge (3 to 4 colour experiments)

Accommodation

Accommodation is not covered, to be arranged by participants. Hotel suggestions: CAMPANILE, 360 avenue du Général Leclerc, 92140 Clamart; LA Roseraie, 32 Avenue Jeanne et Maurice Dolivet, 92260 Fontenay-aux-Roses; Grand Hotel, 51 avenue Aristide Briand, 92350 Le Plessis Robinson.

Travel costs are also not included.

Comments

The course can be well combined with M6 (CyTOF) training module.

Weblink

http://www.idmitcenter.fr/technologies/flowcytech/

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