M7  Flow Cytometry

Course content

DAY-1 (9:00 am to 5:30 pm)

1. Introduction to the course

2. Optimization of protocols for intracellular staining of immune cells (ICS): from the choice of the markers to controls

3. Presentation of the ICS protocol and ICS hands on session Cell stimulation Fixation and Permeabilization

4. Sample Acquisition Course Introduction to DIVA Introduction to FlowJo Quality controls Staining and Acquisition

 

DAY-2 (8:30 am to 12:30)

5. Data analysis hands on session Sample analysis using FlowJo Post sample analysis using SPICE

6. Storage, analysis and visualization of Flow Cytometry data

Learning Outcomes 

To master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.

Course Goals 

The course will comprise lectures of experts in the field presenting methods for assessing the immune response in human and animal models. In particular, it will be focused on combined staining of surface markers and intracellular markers (cytokines and degranulation markers), allowing the characterization of activation, differentiation and functionality of lymphoid and myeloid cells. It will include approaches to master multi-dimensional flow cytometry from the basic theory to data analysis, and biological interpretation of the results.

Timeline 

Application Deadline:  

1 May 2019 / 1 May 2021

Course Dates:

18-19 September 2019/Q3 2021 (1,5 days)

Contact 

Dr. Anne-Sophie Gallouet

anne-sophie.gallouet@cea.fr