Protein and Peptide Chemistry Facility

Protein and Peptide Chemistry Facility - Department of Biochemistry - University of Lausanne, Epalinges, Switzerland

The mission of the Protein and Peptide Chemistry Facility (PPCF) is to provide information  on selected aspects of peptide synthesis and protein structure and to create the resources to investigators to extend their capabilities on peptide synthesis, peptide/protein purification and mass spectroscopic analysis for basic and clinical research.

One of the main lines of activities is the development of synthetic therapeutic peptide vaccines for infectious diseases and for oncology. We use state of the art synthetic peptide chemistry in conjunction with supramolecular chemistry to identify target epitopes and to reconstruct them as effective immunogens.

The PPCF is a research and service laboratory specialized in custom peptide synthesis and the analysis of proteins and peptides. We provide custom peptide synthesis: design and production of any peptide length and complexity (modified peptides) from miligram to multigram quantities.


Equipment:
Automated peptide synthesis is performed using a large scale peptide synthesizer CS136XT from CSBio with UV Fmoc monitoring for scales from 0.5-5 mmol, and two CS366X (scales from 0.01-0.5 mmol) with two reaction vessels each and UV monitoring dedicated for the synthesis of long peptides.
Other peptide synthesizers inculde: one Applied Biosystems 431A and two 433A peptide syntesizers (up to 0.2 mmol), an ACT348 OMEGA Advanced ChemTech multiple synthesizer (0.025 and 0.1 mmol), two Intavis microscale synthesizers, ResPep and MultiPep, both employing Fmoc chemistry, and an Intavis Slide spotter for spotting peptide arrays onto glass slides.
High Performance Liquid Chromatography (HPLC) is performed by three Waters HPLC systems for analytical and preparative HPLC and a Hewlett Packard HPLC system for analytical applications.
Mass spectroscopic analysis is performed by a Voyager-DE Biospectrometry Workstation by Applied Biosystems and a 4700 Proteomics Analyzer MALDI- TOF/TOF by Applied Biosystems.
A Pico Tag hydrolysis system and Waters analytical HPLC are used for amino acid composition analysis.

Users will send the sequence to be synthesized together with the quantity and purity needed. In addition, information will be requested on the arrangements of cysteine residues to allow the correct folding of the material if desired. Furthermore, labelling of products with chromophores, biotin or other modifying agents will be possible.
The users can contact anytime the PPCF chief to discuss the various aspects of the synthesis, purification or analytical characterization of the product by telephone, e-mail or personal visit to the facilities.
Peptides will be delivered with all the documents attesting the completion of the synthesis, the purification and the analytical characterization.

On-site access

No training is available but the PPCF can whenever requested provide physical access to its facilities to external users.

Modalities of access

Organisation short name: PPCF-UNIL

Unit of access: The unit of access is a peptide. A peptide is defined by its sequence, purity and quantity.

Quantity of access available: A maximum of 20 studies are offered.

Timelines: 4-6 weeks depending on the peptide length, sequence and percentage of purity required.

Overview of access provided between January 2011 (1st call) and January 2013 (9th call):
Estimated number of users/User groups: -
Estimated number of projects: -
Access already provided: 0 (This service has been included to the TRANSVAC network in July 2012)                   
Access still available: 20

Last call submission date scheduled for: April 2013 (Call number: 10)

Input – sample requirements

The users will be required to provide the peptide sequence, the percent purity and the quantity they need.

Output – deliverables to User

The peptide is sent in a lyoplilized form together with the quality control documentation (sequence, theoretical molecular weight, analytical HPLC and mass spectrometry data).

Contact

Dr Catherine Servis

ekaterini [dot] servi [at] unil [dot] ch

+41 21 692 5707
+41 21 692 5687